Table 2.
The sum peptide-to-protein corrections per LC–ESI–MS/MS run from the MASCOT, OMSSA, X!TANDEM and SEQUEST algorithms over the experimental treatments of preserved and degraded human EDTA plasma
| TreatmentName | Sum.Correlations | N | CorrelationsPerRun |
|---|---|---|---|
| FD-20 °C | 346,290 | 12 | 28,858 |
| − 80 °C | 254,832 | 7 | 36,405 |
| LN | 401,209 | 11 | 36,474 |
| FDRT | 280,050 | 12 | 23,338 |
| ICE_INHIB_1 h | 42,197 | 2 | 21,099 |
| ICE_INHIB_4 h | 44,270 | 2 | 22,135 |
| ICE_INHIB_8 h | 90,814 | 4 | 22,704 |
| ICE_INHIB_24 h | 102,271 | 4 | 25,568 |
| ICE_INHIB_48 h | 80,709 | 4 | 20,177 |
| ICE_INHIB_72 h | 77,590 | 3 | 25,863 |
| ICE_1 h | 49,197 | 2 | 24,599 |
| ICE_4 h | 74,232 | 3 | 24,744 |
| ICE_8 h | 66,377 | 2 | 33,189 |
| ICE_24 h | 115,975 | 4 | 28,994 |
| ICE_48 h | 107,433 | 5 | 21,487 |
| ICE_72 h | 113,023 | 3 | 37,674 |
| RT_1 h | 538,873 | 12 | 44,906 |
| RT_4 h | 351,457 | 12 | 29,288 |
| RT_8 h | 327,895 | 10 | 32,790 |
| RT_12 h | 435,866 | 12 | 36,322 |
| RT_24 h | 507,283 | 12 | 42,274 |
| RT_48 h | 349,692 | 10 | 34,969 |
| RT_72 h | 403,881 | 11 | 36,716 |
| RT_96 h | 227,777 | 6 | 37,963 |
The redundant correlations made by the sum of all four algorithms to any human protein are shown. Abbreviations: FD-20 °C, Freeze Dried and stored in an electric freezer at − 20 °C for 1 year; − 80 °C, fresh plasma stored in an electric freezer at – 80 °C for 1 year; LN, fresh plasma stored in liquid nitrogen for 1 year; FDRT, freeze dried and stored at room temperature for 1 year; ICE_INHIB, fresh plasma stored on ice with a cocktail of protease inhibitors; ICE, fresh plasma stored on ice; RT, room temperature