Figure 5.

Heat shock protein 60 (HSP60; 5 μg/mL)‐induced dendritic cells (DCs) were cocultured with T cells in 24‐well trans‐well culture plates. T cells 4×10⁵ (upper well) in 200 μL were cultured in the upper chamber while autologous HSP60‐stimulated DCs 2×10⁵ (bottom well) in 500 μL were in the bottom chamber. In parallel, DCs induced in the same condition were cocultured with T cell in same well as mix coculture. T‐cell activation from trans‐well culture was weak in comparison to mix culture. One of 2 individual experiments is represented here (A). HSP60‐induced DCs were cocultured with CD3⁺ T cells in presence or absence of 8 μg/mL anti‐HLA II antibodies (HLA‐II‐blocking antibodies). DCs‐mediated T‐cell activation was inhibited in presence of HLA II antibodies (B). Experiments were performed 3 times and 1 of them is shown as a representative, except for CD69 where inhibition by HLA‐II‐blocking antibodies was nonsignificant in some experiments. *P≤0.05; **P≤0.01; ***P≤0.0001.