Skip to main content
. 2017 Oct 28;6(11):e007030. doi: 10.1161/JAHA.117.007030

Figure 4.

Figure 4

MYL4 p.E11K causes atrial fibrosis and cardiomyocytes apoptosis. A and B, Atrial fibrosis obtained by Masson staining was averaged for 4 to 5 hearts per group (6–10 fields per heart for each group). C and D, Representative images and mean data for TUNEL staining of atrial sections (4–5 hearts per group, 6–10 fields per heart for each group) from WT,MYL4 E11K +/−, and MYL4 E11K +/+rats. E, Confocal immunofluorescence showed that TUNEL‐positive cells costained with α‐actin, indicating that they were cardiomyocytes. TUNEL‐positive staining is green. Nuclei are stained with DAPI (blue). Myocytes are stained for α‐actin (red). Arrows indicate TUNEL‐positive nuclei. Values are mean±SEM. *P<0.05; **P<0.01; ***P<0.001 vs WT;, # P<0.05 among 15 days, 1 month, 3 months, and 6 months rats. DAPI indicates 4′,6‐diamidino‐2‐phenylindole; LA, left atrial; MYL4, myosin light‐chain 4; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling; WT, wild type.