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. 2017 Sep 28;6(10):e006421. doi: 10.1161/JAHA.117.006421

Figure 5.

Figure 5

Chronic stress stimulates inflammatory action in ischemic muscles of stressed mice. A, Immunohistochemical staining was performed using antibodies against macrophages (Mac‐3) in ischemic and nonischemic muscles of nonstressed and stressed mice on d 4 after ischemia. Representative images and quantitative data show infiltrated numbers of macrophages (n=6, ANOVA and Tukey's post hoc tests). B and C, Gelatin zymography assay was performed in ischemic and nonischemic muscles of nonstressed and stressed mice on d 4 after ischemia. Representative images and quantitative data show the levels of the MMP‐2 and MMP‐9 gelatinolytic activities (n=3, ANOVA and Tukey's post hoc tests). Values are mean±SE. *P<0.01 vs corresponding controls. Scale bar: 50 μm. MMP‐2 indicates matrix metalloproteinase‐2; NS, not significant.