Fig. 2.

Dot-blot assay for transglycosylase activity of enzyme extracts of young and old Zygnema S and Klebsormidium crenulatum filaments. Test papers were coated with 1% (w/v) solutions of different cell wall polysaccharides and ∼5 mM XyGO-SR. Enzyme extracts (in 5 µl aliquots), were loaded on the test papers and incubated for 2 h before washing with an ethanol:formic acid:water (1:1:1, v/v/v) mixture for 2 h, rinsing twice with distilled water and drying overnight. XyGO-SR was visualized at 365 nm. Young filaments are 1 month old; old filaments are 12 months old.