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. 2018 Jan 15;131(2):jcs203208. doi: 10.1242/jcs.203208

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© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Fluorescence microscopy images showing integration of the fluorescent acceptor substrate XyGO-SR into Chara sp. cell walls. After incubation in XyGO-SRs, Chara was washed in culture medium and viewed using the DAPI channel of an epifluorescence microscope at ×40 magnification. (A) Bright-field image. (B,C) Incorporation of XyGO-SRs in all cell walls. The walls of the stipulodes, and the cells towards the tip of the main axis and branchlets appeared to have incorporated the most XyGO-SRs and fluoresced the most strongly. (D) Control in which Chara sp. was incubated with non-fluorescent XyGOs.