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. 2018 Jan 15;131(2):jcs203208. doi: 10.1242/jcs.203208

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© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — Whole-cell labelling of Klebsormidium crenulatum. Young (A,C,E,G) and old (B,D,G,H) filaments labelled with the monoclonal antibodies LM15, 400-4, 400-3 or LM10 (green) and visualised with a confocal microscope. The corresponding bright-field images include red chloroplast autofluorescence. (A) Filament with weak staining in restricted areas. (B) Intense labelling in thickened cell corners between individual cells and some staining in cross cell walls. (C) Staining in outer cell walls. (D) Intense staining in cell corners and thickened cross cell walls. (E,F) Staining in cell corners (arrowheads) and occasionally in longitudinal cell walls of longer cells. (G) Punctuate staining pattern in outer cell walls. (H) Similar appearance to cells shown in G; additionally, cross cell walls show staining (arrows). Young filaments are 1 month old; old filaments are 12 months old. Scale bars: 10 µm.