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Viscometric assay estimating the hydrolysis of four cell wall polysaccharides by enzyme extracts of young and old Zygnema S and Klebsormidium crenulatum filaments. Reaction mixtures contained 1% (w/v) polysaccharide, 300 mM Na succinate (pH 5.5) and 10% (v/v) dialysed algal enzyme extract. (A) Xyloglucan, (B) galactomannan, (C) mixed-linkage (1→3,1→4)-β-glucan (MLG) from oat, (D) xylan. n=3 (s.d. <5%). Young filaments are 1 month old; old filaments are 12 months old.
