Figure 7.

CGL/H₂S Is Required for Negative Regulation of TH and GH/IGF-I Signaling

(A and B) Western blot analysis of H₂S-producing enzymes CGL, CBS, and 3MST (n = 3) (A) and H₂S production capacity in livers of CGL WT and KO mice on Normal and PTU diets (n = 4–5) (B). The asterisk indicates the significance of the difference relative to CGL WT mice on the Normal diet; ^∗p < 0.05.

(C) Serum T4 levels over a 3-week time course of Normal versus PTU diets in CGL WT and KO mice as indicated (n = 4–5).

(D) Pituitary mRNA expression of TSHα and TSHβ in CGL WT and KO mice after 3 weeks of Normal or PTU diet (n = 4–5). The asterisk indicates the significance of difference between diets within genotype, and the # sign indicates the significance of difference between CGL WT and CGL KO mice on the PTU diet; ^∗/^#p < 0.05.

(E) Serum TSH levels after 3 weeks of Normal or PTU diet in CGL WT and KO mice (n = 4–5). The asterisk indicates the significance of difference between diets within genotype, and the # sign indicates the significance of difference between CGL WT and CGL KO mice on the PTU diet; ^∗/^#p < 0.05.

(F) Percent change in serum T4 levels after 3 days of fasting in CGL WT and CGL KO mice (n = 3–4). The asterisk indicates the significance of the difference between day 0 and day 3 T4 levels; ^∗p < 0.05.

(G–I) Pituitary GH mRNA expression (n = 4–5) (G), liver GHR and IGF-I mRNA expression (n = 4–5) (H), and serum IGF-1 (n = 4–5) (I) in CGL WT and KO mice after 3 weeks on a Normal or PTU diet as indicated. The asterisk indicates the significance of the difference between diets within genotype, and the # sign indicates the significance of the difference between genotypes on the PTU diet; ^∗/#p < 0.05.

(J) Fold change in serum IGF-I between day 0 and day 3 of a 3-day fast in CGL WT and KO mice (n = 3–4/group). The asterisk indicates the significance of the difference between day 3 and day 0; ^∗p < 0.05.

(K) Serum IGF-1 levels in mice 7 days after adenoviral infection with Ad-Null control or Ad-CGL overexpression adenovirus expressed relative to Ad-Null control (n = 4/group). The asterisk indicates the significance of the difference between Ad-Null and Ad-CGL; ^∗p < 0.05.

(L) Fold change in serum IGF-1 after a 2-day fast in female CGL WT and KO mice with +/−NaHS supplementation in the CGL KOs (n = 4–5/group). The asterisks indicate the significance of the difference between serum IGF-1 levels on day 2 compared with day 0; ^∗p < 0.05.

(M) Relationship between diet, GH/TH, and H₂S production.

Error bars are ± SEM. See also Figure S7.