REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-CGL (Anti-Cystathionase)	Abcam	Ab151769
Anti-CBS	Abcam	Ab135626
Anti-3MST (Anti-MPST)	Sigma	HPA001240
Anti-Stat5	Santa Cruz	Sc-835
Anti-p-Stat5	Cell Signaling Technology	9359
Anti-GNMT	Aviva	ARP43565_P050
Anti-AHCY	Abcam	Ab56146
Anti-ATF4 (Anti-CREB-2)	Santa Cruz	Sc-200
Anti ATG5	Novus	NB110-53818
Anti ATG7	Sigma	A2856
Anti-beta Tubulin	Cell Signaling	2128
Anti-Actin	Cell Signaling	4970
HRP conjugated anti-rabbit	Dako	P044801-2
Bacterial and Virus Strains
Ad-CMV-CGL (Ad-mCTH)	Vector Biolabs	ADV-256305
Ad-CMV-Null	Vector Biolabs	1300
Lentiviral sh-GFP	Laboratory of Dr. Alec Kimmelman	N/A
Lentiviral sh-ATG5	Laboratory of Dr. Alec Kimmelman	N/A
Lentiviral sh-ATG7	Laboratory of Dr. Alec Kimmelman	N/A
Biological Samples
Livers (frozen) taken from experimental mouse strains listed in the Experimental Models: Organisms/Strains section	See Experimental Models: Organisms/Strains section	See Experimental Models: Organisms/Strains section
Serum/Plasma (frozen) taken from experimental mouse strains listed in the Experimental Models: Organisms/Strains section	See Experimental Models: Organisms/Strains section	See Experimental Models: Organisms/Strains section
Chemicals, Peptides, and Recombinant Proteins
NaHS	Sigma	161527
GYY4137	Sigma	SML0100
Lanreotide	Sigma	SML0132
PTU/LID diet	Harlan Teklad	TD 95125
T3 (Triiodo-L—thyronine)	Sigma	T2752
GC-1	Laboratory of Dr. Thomas Scanlan	Chiellini et al., 1998
GH (growth hormone)	Sigma	S8648
FGF21	Genscript	Z03290
Bafilomycin	Sigma	B1793
Chloroquine	Sigma	C6628
DL-Propargylglycine	Sigma	P7888
Aminooxyacetic acid	Sigma	C13408
AZD1480	Selleckchem	S2162
Passive Lysis Buffer (5x)	Promega	E1941
PLP (Pyridoxal 5′-phosphate)	Sigma	P9255
L-cysteine	Sigma	C7352
lead (II) acetate trihydrate	Sigma	316512
P3 H2S Detection Probe	From the lab of Prof. K.H. Ahn	Singha et al., 2015
Critical Commercial Assays
Mouse/Rat IGF-1 ELISA kit	R&D Systems	SMG100
T4 ELISA kit	Diagnostic Automation/ Cortez Diagnostics, Inc	3149-18
MILLIPLEX MAP Mouse Endocrine (TSH) Assay	EMD Millipore	MPTMAG-49K
Experimental Models: Cell Lines
Hepa1-6 2Cl BirA/TRbeta	Laboratory of Dr. Anthony N. Hollenberg	N/A
Hepa1-6 (mouse liver hepatoma)	ATCC	CRL-1830
Primary mouse hepatocytes prepared from C57BL/6 mice (freshly isolated in the lab of Dr. James Mitchell for each experiment)	Jackson Laboratories and laboratory of Dr. James R. Mitchell	000664 and this paper
ATG5 Knockout mouse embryonic fibroblasts	From the laboratory of Dr. Gokhan Hotamisligil	Yang et al., 2010
ATG7 Knockout mouse embryonic fibroblasts	From the laboratory of Dr. Gokhan Hotamisligil	Yang et al., 2010
Primary CGL WT and KO mouse tail dermal fibroblasts	From the laboratory of Dr. James R. Mitchell	This paper
Experimental Models: Organisms/Strains
129/C57BL/6 background WT and KO CGL Male and Female Mice	Laboratories of Dr. Rui Wang and Dr. James R. Mitchell	Hine et al., 2015, Yang et al., 2008
WT and LirKO Female Mice	Laboratory of Dr. James R. Mitchell	Harputlugil et al., 2014
Male and Female WT and Snell Dwarf mice	Laboratory of Dr. Richard Miller	Dozmorov et al., 2002
Female WT and Ames Dwarf mice	Laboratory of Dr. Andrzej Bartke	Panici et al., 2010
Male and Female WT and GHRKO Mice	Laboratory of Dr. Richard Miller	Wang and Miller, 2012
Male and Female WT and IRS-1 KO mice	Laboratory of Dr. Colin Selman	Selman et al., 2008
Male WT and FGF21 overexpressing mice	Laboratory of Dr. Pavlos Pissios	Kharitonenkov et al., 2005
TRbeta NI/NI, NI/PV, and mutant PV/PV mice	Laboratory of Dr. Sheu-yann Cheng	(Kato et al., 2004
C57BL/6 mice	Jackson Laboratories	000664
B6D2F1 hybrid mice	Jackson Laboratories	100006
Oligonucleotides
IGF-1 F: TGCTTGCTCACCTTCACCA IGF-1 R: CAACACTCATCCACAATGCC	N/A	N/A
GHR F: ATTCACCAAGTGTCGTTCCC GHR R: TCCATTCCTGGGTCCATTCA	N/A	N/A
CGL F: TTGGATCGAAACACCCACAAA CGL R: AGCCGACTATTGAGGTCATCA	N/A	N/A
CBS F: GGGACAAGGATCGAGTCTGGA CBS R: AGCACTGTGTGATAATGTGGG	N/A	N/A
HPRT F:TTTCCCTGGTTAAGCAGTACAGCCC HPRT R:TGGCCTGTATCCAACACTTCGAGA	N/A	N/A
RPL13 F:TTCGGCTGAAGCCTACCAGAAAGT RPL13 R:TCTTCCGATAGTGCATCTTGGCCT	N/A	N/A
MAT1A F: GATAGCAGATCTGAGGCGCT MAT1A R: TGCACCATTATCCTGCATGT	N/A	N/A
GNMT F: AAGAGGGCTTCAGCGTGATG GNMT R: CTGGCAAGTGAGCAAAACTGT	N/A	N/A
AHCY F: CGCCAGCATGTCTGATAAAC AHCY R: CCTGGCATCTCATTCTCAGC	N/A	N/A
BHMT F: TTAGAACGCTTAAATGCCGGAG BHMT R: GATGAAGCTGACGAACTGCCT	N/A	N/A
For a full list of all primers used, please seeTable S1
Software and Algorithms
ImageJ	National Institutes of Health	Windows version, https://imagej.nih.gov/ij/download.html