Table 2.
Isolate | MIC (μg/mL) |
ESBL genes | MBL genes | Aminoglycoside-Modifying Enzyme Genes | Relative expression levels |
Inserted into oprD | ISPpu21/oprD | RAPD-PCR | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CO | GEN | AK | IMP | MEM | CAZ | CIP | mexX | mexB | mexD | mexT | oprD | |||||||
PA11 | ≤2 | 256 | 64 | 8 | 64 | 128 | 16 | blaTEM | − | aadA- aadB- rmtA-armA | 1.2 | 1.1 | 21.11 | ND | 12.22 | + | + | A |
PA17 | ≤2 | 128 | 32 | 4 | 128 | 128 | 32 | blaTEM | − | aadA- aadB-rmtA | 2.1 | 1.2 | 35.17 | 0.97 | 10.25 | + | + | A |
PA22 | ≤2 | 32 | 32 | 256 | 64 | 128 | 16 | blaTEM | − | aadA- aadB- rmtA-armA | 1.3 | 1.1 | 1.7 | 0.89 | 0.57 | + | + | A |
PA31 | ≤2 | 32 | 32 | 256 | 128 | 128 | 128 | blaTEM | − | aadA- aadB-armA | 1.2 | 1.3 | 4.56 | 0.35 | 1.37 | + | + | A |
PA42 | ≤2 | 256 | 256 | 256 | 128 | 64 | 32 | blaTEM | − | aadA- aadB- rmtA-armA | 1.2 | 1.1 | 19.2 | 8.22 | 30.36 | + | + | A |
PA43 | ≤2 | 256 | 256 | 256 | 128 | 128 | 32 | blaTEM | − | aadA- aadB- rmtA-armA | 1.3 | 1.1 | 4.22 | 2.07 | 16.14 | + | + | A |
PA46 | ≤2 | 256 | 256 | 256 | 8 | 64 | 4 | blaTEM | − | aadA- aadB- rmtA-armA | 0.18 | 1.2 | 15.24 | 2.31 | 25.56 | + | + | C |
PA48 | ≤2 | 128 | 256 | 4 | 8 | 256 | 8 | blaTEM | − | aadA- aadB- rmtA-armA | 0.53 | 1.2 | 23.57 | 1.06 | 9.01 | + | + | A |
PA49 | ≤2 | 32 | 256 | 4 | 8 | 16 | 16 | blaTEM | − | aadA- rmtA | 1.4 | ND | ND | ND | 4.07 | + | + | A |
Psa6 | ≤2 | 256 | 256 | 4 | 4 | 4 | 16 | blaTEM | − | aadA- rmtA- | 1.10 | 3.68 | 2.21 | 2.36 | 35.01 | + | + | B |
Abbreviations: GEN, gentamicin; AK, amikacin; IMP, imipenem; MEM, meropenem; CAZ, cefotaxime; CIP, ciprofloxacin; ESBL, extended-spectrum β-lactamases; MBL, metallo-β-lactamases; RAPD-PCR, random amplified polymorphic DNA polymerase chain reaction.
–, Negative; +, Positive; ND, not determined.