Table 2.

Antibiotic susceptibility, PCR results for genes and gene expression data for ISPpu21-carrying Pseudomonas aeruginosa isolates

Isolate	MIC (μg/mL)							ESBL genes	MBL genes	Aminoglycoside-Modifying Enzyme Genes	Relative expression levels					Inserted into oprD	ISPpu21/oprD	RAPD-PCR
	CO	GEN	AK	IMP	MEM	CAZ	CIP				mexX	mexB	mexD	mexT	oprD
PA11	≤2	256	64	8	64	128	16	blaTEM	−	aadA- aadB- rmtA-armA	1.2	1.1	21.11	ND	12.22	+	+	A
PA17	≤2	128	32	4	128	128	32	blaTEM	−	aadA- aadB-rmtA	2.1	1.2	35.17	0.97	10.25	+	+	A
PA22	≤2	32	32	256	64	128	16	blaTEM	−	aadA- aadB- rmtA-armA	1.3	1.1	1.7	0.89	0.57	+	+	A
PA31	≤2	32	32	256	128	128	128	blaTEM	−	aadA- aadB-armA	1.2	1.3	4.56	0.35	1.37	+	+	A
PA42	≤2	256	256	256	128	64	32	blaTEM	−	aadA- aadB- rmtA-armA	1.2	1.1	19.2	8.22	30.36	+	+	A
PA43	≤2	256	256	256	128	128	32	blaTEM	−	aadA- aadB- rmtA-armA	1.3	1.1	4.22	2.07	16.14	+	+	A
PA46	≤2	256	256	256	8	64	4	blaTEM	−	aadA- aadB- rmtA-armA	0.18	1.2	15.24	2.31	25.56	+	+	C
PA48	≤2	128	256	4	8	256	8	blaTEM	−	aadA- aadB- rmtA-armA	0.53	1.2	23.57	1.06	9.01	+	+	A
PA49	≤2	32	256	4	8	16	16	blaTEM	−	aadA- rmtA	1.4	ND	ND	ND	4.07	+	+	A
Psa6	≤2	256	256	4	4	4	16	blaTEM	−	aadA- rmtA-	1.10	3.68	2.21	2.36	35.01	+	+	B

Abbreviations: GEN, gentamicin; AK, amikacin; IMP, imipenem; MEM, meropenem; CAZ, cefotaxime; CIP, ciprofloxacin; ESBL, extended-spectrum β-lactamases; MBL, metallo-β-lactamases; RAPD-PCR, random amplified polymorphic DNA polymerase chain reaction.

–, Negative; +, Positive; ND, not determined.