Table 1. Response of transgenic mice and corresponding wt controls to mouse scrapie.
| Inoculum | Genotype of recipient mice | ||
|---|---|---|---|
| 129/Sv-C57/BL6 | TgPrP(TetraH>G) | TgPrP(H95G) | |
| Primary passage | |||
| RML | 150 ± 12 (n = 8) | Line 34: 347 ± 28 (n = 8) | Line 4: 116 ± 3 (n = 8), Line 11: 87 ± 3 (n = 8), Line 13: 89 ± 8 (n = 8) |
| Secondary passage | |||
| TgPrP(TetraH>G)-passaged RML, line HG34 animal #258 | 160 ± 8 (n = 6) | Line 34: 427 ± 12 (n = 8) | Not determined |
| TgPrP(TetraH>G)-passaged RML, line HG34 animal #260 | 153 ± 5 (n = 6) | Line 34: 372 ± 27 (n = 5) | Not determined |
| TgPrP(H95G)-passaged RML, line 13 animal #304 | 150 ± 8 (n = 6) | Not determined | Line 13: 75 ± 1 (n = 6) |
| TgPrP(H95G)-passaged RML, line 13 animal #305 | 152 ± 4 (n = 6) | Not determined | Line 13: 77 ± 4 (n = 8) |
Mean incubation times ± standard deviation in days after i.c. inoculation (dpi) are presented. For primary transmission mice expressing full-length mouse wt PrP (129/Sv-C57/Bl6) or mutant PrP (PrP(TetraH>G) and PrP(H95G)) were i.c. inoculated with 30 μl of 10% (wt/vol) RML homogenate in PBS. For secondary passage, brain homogenates from different terminally ill TgPrP(TetraH>G) (line HG34, mouse #258 and 260) as well as TgPrP(H95G) mice (line 13, mouse #304 and 305) were i.c. inoculated in the same lines of recipient mice.