Skip to main content
. 2017 Dec 8;12(12):e0189331. doi: 10.1371/journal.pone.0189331

Fig 2. PsrA positively controls pqsA.

Fig 2

(A) qRT-PCR was performed on RNA from strains PAO1 and PGW-ΔpsrA to analyze the relative expression of lasR, pqsH, pqsR and pqsA. Data are presented as average fold change ± SD in the psrA mutant compared to the wild type strain (set to a value of 1). The gene clpX was used as a reference gene to normalize expression and each experiment was completely repeated three times. (B and C) PQS production by strains PAO1 or PGW-ΔpsrA expressing (B) PQS synthetic genes or (C) RpoS. Cultures were grown for 24 h in LB medium supplemented with 0.5% L-arabinose to induce genes. PQS was then extracted and quantified as described in Materials and Methods. Data are presented as the average ± SD of three independent experiments. (B) Plasmids contained by strains are: open bars, control vector; solid bars, pqsABCD expression vector; and hatched bars, pqsH expression vector. (C) Presence of the rpoS expression vector is indicated by a plus (+) symbol.