Table 1. Bacterial strains and plasmids used in this study.
| Strain or Plasmid | Relevant genotype or phenotype | Reference or source |
|---|---|---|
| E. coli DH5α | λ- φ80dlacZΔM15 Δ(lacZYA-argF) U196 recA1 endA1 hsdR17 (rK- mK-) supE44 thi-1 gyrA relA1 | [23, 24] |
| P. aeruginosa strains | ||
| PAO1 | Wild type | [25] |
| PGW-ΔpsrA | psrA deletion in PAO1 | This Study |
| PGW-ΔPA0506 | PA0506 deletion PAO1 | This Study |
| PGW-ΔpsrAΔPA0506 | PA0506 deletion in PGW-ΔpsrA | This Study |
| PGW-ΔPA0507 | PA0507 deletion in PAO1 | This Study |
| PGW-ΔpsrAΔPA0507 | PA0507 deletion in PGW-ΔpsrA | This Study |
| PGW-ΔPA0508 | PA0508 deletion in PAO1 | This Study |
| PGW-ΔpsrAΔPA0508 | PA0508 deletion in PGW-ΔpsrA | This Study |
| Plasmids | ||
| pHERD20T | E. coli/P. aeruginosa shuttle expression vector | [26] |
| pEX18Ap | Suicide vector for P. aeruginosa | [27] |
| pEX1.8 | Expression plasmid | [28] |
| pMW105 | rpoS in pEX1.8 | [29] |
| pPQSsynOE | PBAD-pqsABCD on pHERD20T | [30] |
| pGW-1 | psrA deletion in pEX18Ap | This Study |
| pGW-2 | PBAD-psrA on pHERD20T | This Study |
| pGW-14 | PsrA-6HIS in pHERD20T | This Study |
| pGW-15 | PA0506 deletion in pEX18Ap | This Study |
| pGW-16 | PA0507 deletion in pEX18Ap | This Study |
| pGW-18 | PBAD-PA0507 on pHERD20T | This Study |
| pGW-19 | PA0508 deletion in pEX18Ap | This Study |
| pGW-20 | PBAD-PA0508 on pHERD20T | This Study |
| pGW-21 | PBAD-PA0506 on pHERD20T | This Study |
| pGW-22 | PBAD-fadAB5 on pHERD20T | This Study |