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. 2017 Dec 8;12(12):e0189017. doi: 10.1371/journal.pone.0189017

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© 2017 Hornum et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Double immunofluorescence staining for C5aR (red signal A—F) and either CD68⁺ macrophages (green signal A, B and C), CD3⁺ T cells (green signal in D), or MPO⁺ neutrophils (green signal in E and F) of the synovium from rheumatoid arthritis patients undergoing joint replacement (A, B, D and E) or synovectomy (C and F), analysed by confocal microscopy (A, B, D and E) and epifluorescence microscopy (C and F). Framed area in E is seen in B. Note the co-localization of C5aR and CD68⁺ macrophages in A, B and C, and MPO⁺ neutrophils F, and lack of co-localization with a CD3⁺ T cell in D. The nuclei (in blue) were counterstained with Hoechst. Bars: (A) 100 μm, (B and D) 5 μm, (E) 500 μm, (C and F) 50 μm.