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. 2017 Aug 4;8(57):96482–96495. doi: 10.18632/oncotarget.19946

Figure 2. PRKCI amplification and overexpression in CCOC cells and patient specimens.

Figure 2

A. Copy number alterations in human chromosome 3 of five CCOC cell lines indicate amplification at 3q26 region, containing PRKCI gene. B. Correlation between PRKCI DNA copy number, mRNA expression and protein expression in 12 clear cell ovarian cancer cell lines. Relative PRKCI copy number, mRNA expression level and protein expression level of 12 clear cell ovarian cancer cell lines were determined by qPCR, qRT-PCR and Western blot respectively. The correlation was determined by Pearson correlation coefficient. C. PRKCI protein expression level of 12 CCOC cell lines and 2 normal ovarian surface epithelium cell lines (IOSE-80 and IOSE-120) as determined by Western blot analysis. D. Copy number variation qPCR on 27 CCOC tumor specimen. Relative PRKCI copy number was calculated by comparing normal and clear cell cancer specimen with pooled normal reference genomic DNA. E. Microarray and qRT-PCR data of PRKCI mRNA expression level by comparing 10 CCOC tumor specimens with 10 normal ovarian surface epithelium samples. F. Immunohistochemical staining of PRKCI protein expression in an additional 40 CCOC tumor tissues. Representative pictures are shown to demonstrate PRKCI intensity of (i) 0, (ii), 1+, (iii) 2+, (iv) 3+. Original magnification x200. G. Kaplan-Meier estimates of survival of the 40 CCOC patients according to the PRKCI expression level in cancer tissue with immunohistochemical staining. Analysis was done by median cut with the p-value of log-rank test. Blue line, samples with low PRKCI ( < median); red line, samples with high PRKCI (≥median). Patients with high expression of PRKCI showed lower percentage survival compared with patients with low expression of PRKCI.