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. 2017 May 20;8(57):96697–96709. doi: 10.18632/oncotarget.18043

Figure 1. Stem-like properties of BTSCs and its cell viability assessment after the treatment with riluzole.

Figure 1

(A) BTSCs stained with anti-CD133 und anti-Nestin antibodies, known neural stem and progenitor cell markers, in green and DAPI in blue. (B) Cell viability obtained by MTT assay (n = 5; after 48 and 72 h) after the treatment with 10 µM and 50 µM riluzole alone or in combination with (C) irradiation of 5 Gy (n = 3; after 72 h) or (D) in combination with 200 µM TMZ and irradiation of 5 Gy (n = 3; after 72 h). (E) A decrease in Mcl-1 protein expression as a consequence of riluzole action was presented by representative western blot with anti-Mcl-1 antibody 72 h after the treatment as well as by densitometry analysis of three independent experiments. Western blot with anti-LC3B antibody shows an increase in LC3B-II and indicates autophagy as a form of cell death. A statistical analysis was performed using two-sided t-tests of three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001). The scale bar is 50 µm.