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. 2017 Jun 13;8(57):96852–96864. doi: 10.18632/oncotarget.18451

Figure 1. Mouse Lewis lung cancer cell line culture contains a spheroid-forming subcomponent.

Figure 1

(A) the morphology of spheroid forming cell lines from mouse Lewis lung cancer cell lines (LLC-SE) and LLC-Parental, bar=120um. (B) the analysis of mRNA expression of embryonic stem cell markers (Oct4, Sox2, Klf4 and c-Myc) in LLC-SE cell lines and LLC-Parental, the tata-Box binding protein gene (Tbp) was reference gene. (C) Flow cytometry of LLC-SE and LLC-Parental for CD133. (D) the analysis of the number of sphers in 6-well plate in one well of which 200 LLC-SE and LLC-Parental were seed, P=0.01. (E) Tumor formation in nude mice in which 10 and 1000 LLC-SE cells were injected respectively, n is the number of nude mice.