Table 2. PCR primer sequence.
Gene name | Forward primers | Reverse primers |
---|---|---|
mouse Oct4 | TTCCCTCTGTTCCCGTCACT | TGTCTACCTCCCTTGCCTTG |
mouse Sox2 | AGGGCTGGGAGAAAGAAGAG | ATCTGGCGGAGAATAGTTGG |
mouse Klf4 | GGACCACCTTGCCTTACACA | GACTTGCTGGGAACTTGACC |
mouse c-Myc | ACAAGAGGCGGACACACAAC | TTGAATGGACAGGATGTAGGC |
mouse Tbp | GCGACCCTCACATCAAACT | CAGTGCCACATACCAACT |
human Oct4 | CGAAAGAGAAAGCGAACCAG | TGAAGTGAGGGCTCCCATAG |
human Sox2 | CACAACTCGGAGATCAGCAA | GTTCATGTGCGCGTAACTGT |
human Nanog | AGATGCCTCACACGGAGACT | TCTGGAACCAGGTCTTCACC |
human Klf4 | TTCTCTCCAATTCGCTGACC | GTACACCGGGTCCAATTCTG |
human c-Myc | TTCGGGTAGTGGAAAACCAG | CAGCAGCTCGAATTTCTTCC |
human Tbp | TATAATCCCAAGCGGTTTGC | CACAGCTCCCCACCATATTC |