Sheet 1 Nucleotide Sequence Specific Primers.
| Gene | Primer sequences | Species | |
|---|---|---|---|
| TLR3 | 5’CCTTGTTGGGACTGTGGC3’ | 5’GGCAGGTGGCAATCTTCT3’ | NMR |
| TLR4 | 5’GAGCCGCTGGTGTATCTT3’ | 5’CAGGGACTTCTCCAACTTTT3’ | NMR |
| IFN-β | 5’ACAACGCAGCAGCAGTTT3’ | 5’GAGCAGCATTCTCCTTCC3’ | NMR |
| TNF-α | 5’ATGGCATGGATCTAACGG3’ | 5’CGGCTGACAGTATGGGTG3’ | NMR |
| GAPDH | 5’CGCCTGCTTCACCACCTT3’ | 5’CCTGCCGCCTGGAGAAA3’ | NMR |
| TLR3 | 5’AGACTGATGCTCAGGAGGGTG3’ | 5’GAAGAGGGCGGAAAGGTG3’ | ICR |
| TLR4 | 5’TTGATACTGACAGGAAACCCTA3’ | 5’TTGTCTCCACAGCCACCA3’ | ICR |
| IFN-β | 5’ATCCCTATGGAGATGACG3’ | 5’ATGGCAAAGGCAGTGTAA3’ | ICR |
| TNF-α | 5’CACCACGCTCTTCTGTCT3’ | 5’ATTTGGGAACTTCTCATCC3’ | ICR |
| GAPDH | 5’CATGGCCTTCCGTGTTCCTA3’ | 5’GCGGCACGTCAGATCCA3’ | ICR |