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. 2017 Oct 13;8(57):97187–97205. doi: 10.18632/oncotarget.21907

Figure 2. Salidroside restores the expression of skeletal muscle cells angiogenic factors repressed by hyperglycemia.

Figure 2

(A, B) Protein expression levels of PHD family in the C2C12 cells treated with salidroside and cultured under hyperglycemia, as determined by western blotting: (A) representative images; and (B) quantification of protein expression levels. (C, D) Protein expression level of HIF-1α in C2C12 cells treated with salidroside and cultured under hyperglycemia, as determined by western blotting: (C) representative images; and (D) quantification of protein expression level. (E, F) Protein expression level of HIF-1α in the PHD3-overexpressing C2C12 cells cultured under hyperglycemia and treated with salidroside, as determined by western blotting: (E) representative images; and (F) quantification of protein expression level. (G–I) Expression levels of VEGF-A and PDGF-BB in C2C12 cells treated with salidroside and cultured under hyperglycemia: (G) mRNA expression levels, as analyzed by quantitative RT-PCR (qPCR); (H) protein expression levels, as examined by western blotting; and (I) quantification of protein expression levels. (J) Secretion levels of VEGF-A (left panel) and PDGF-BB (right panel) from C2C12 cells treated with salidroside and cultured under hyperglycemia, as analyzed by ELISA. (K–M) Expression levels of VEGF-A and PDGF-BB in the PHD3-overexpressing C2C12 cells cultured under hyperglycemia and treated with salidroside: (K) mRNA expression levels, as quantified by qPCR; (L) protein expression levels, as examined by western blotting; and (M) quantification of protein expression levels. All experiments were done under hypoxia, and cells cultured under low glucose condition or cells transfected with pcCon and treated with PBS were used as controls. β-Actin was used for normalization in qPCR, and as a loading control in western blotting. Quantification data were shown as relative to that of control, and expressed as mean ± S.D. (n = 3 from three independent experiments). **P < 0.01; Low: low glucose; High: high glucose; SA: salidroside.