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. 2017 Oct 13;8(57):97187–97205. doi: 10.18632/oncotarget.21907

Figure 6. Salidroside enhances endothelial cells proliferation and migration potentials by suppressing hyperglycemia-induced skeletal muscle cells PHD3 accumulation.

Figure 6

(A, B) The proliferation of HUVECs cultured with indicated conditioned media, as analyzed by Ki67 staining: (A) representative images (scale bars: 200 μm); and (B) quantification of the ratio of Ki67 positive cells to DAPI positive cells. (C, D) The mobility of HUVECs cultured with indicated conditioned media, as examined by transwell chamber assay: (C) representative images (scale bars: 100 μm); and (D) quantification of migrated cells. (E, F) Morphological changes of F-Actin, as determined by phalloidin staining: (E) representative images (scale bars: 100 μm for upper panels and 50 μm for lower panels), lower panels showed the enlarged images of the cropped part in the upper panels; (F) quantification analysis of fractal dimension. Conditioned media were collected from C2C12 cells transfected with pcCon or pcPHD3, treated with PBS or salidroside and cultured under hyperglycemia. All experiments were done under hypoxia. Data shown are representative from three independent experiments. Quantification data were expressed as mean ± S.D. (n = 6). **P < 0.01; pcCon: pcDNA3.1(+); SA: salidroside.