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. 2017 Oct 13;8(57):97187–97205. doi: 10.18632/oncotarget.21907

Figure 9. Salidroside promotes blood perfusion recovery by suppressing PHD3 and promoting angiogenic factors expression in the gastrocnemius muscle of diabetic HLI model mice.

Figure 9

(A–C) The expression levels of PHD family in the gastrocnemius muscle of the ischemic hind limb of diabetic HLI model mice treated with salidroside or PBS: (A) mRNA expression levels, as examined by quantitative RT-PCR (qPCR); (B) representative images of protein expression levels, as examined by western blotting; and (C) quantification of protein expression levels. (D–F) The expression levels of VEGF-A and PDGF-BB in the gastrocnemius muscle of the ischemic hind limb of diabetic HLI model mice treated with salidroside or PBS: (D) mRNA expression levels, as examined by qPCR; (E) representative images of protein expression levels, as examined by western blotting; and (F) quantification of protein expression levels. β-Actin was used for normalization in qPCR, and as a loading control in western blotting. Quantification data were shown as relative to that of control, and expressed as mean ± S.D. (n = 3 from three independent experiments). NS: not significant; **P < 0.01; Con: animals treated with PBS; SA: animals treated with salidroside.