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. 2017 Oct 19;8(57):97246–97259. doi: 10.18632/oncotarget.21896

Figure 4. FUT2 knockdown inhibits migration and invasion of lung adenocarcinoma cells.

Figure 4

(A) FUT2 and GAPDH amplification curve and melting curve, and the relative FUT2 mRNA level. (B) The FUT2 protein expression in the A549 cells by Western Blot analysis, GAPDH was acted as a loading control, the intensity was evaluated using Image J computer software. (C) The expression of FUT2 protein in the H1299 cells by Western Blot analysis, GAPDH was acted as a loading control, the intensity was evaluated using Image J computer software. (D) Cell migration was evaluated by transwell assay. (E) Cell invasion ability was evaluated by matrigel transwell assay. A549: untreated group, NC: transfected scramble vector into lung adenocarcinoma cells, RNAi-FUT2: transfected sh-FUT2 vector into lung adenocarcinoma cells, H1299: untreated group, *P < 0.05, **P < 0.01, ***P < 0.001. Data are presented as means ± SEM, significant difference between groups as indicated.