Table 3. Microparticulosome signature based on a color code: high (red) and low (green) levels of MPs (MPs/ μL of plasma), according to the occurrence or not of a thrombo-embolic event, in colorectal and pancreatic cancers.
| Colorectal cancer | Pancreatic cancer | |||||
|---|---|---|---|---|---|---|
| Thrombo-embolic event | No thrombo embolic event | Thrombo-embolic event | No thrombo embolic event | |||
| n | 10 | 74 | P value | 9 | 25 | P value |
| Total MPs |
7532
(a) [1157–16942] (100%) |
5540 [865–53956] (100%) |
0.58 |
11920 [1855–2119000] (100%) |
6544 [1679–355459] (100%) |
0.39 |
| Subpopulations | ||||||
| PMPs |
4293 [856–11775] (57%)(b) |
3527 [822–45959] (64%) |
0.69 |
5131 [110–32515] (43%) |
3558 [288–44275] (57%) |
0.39 |
| EMPs |
8 [1.1-29] (0.1%) |
2 [0.6-836] (0.04%) |
0.52 |
102 [1.3-1507] (0.9%) |
45 [1–2340] (0.7%) |
0.33 |
| EryMPs |
113 [0–6641] (1.5%) |
264 [0–13026] (5%) |
0.40 |
448 [0–3832] (4%) |
207 [14–8155] (3%) |
0.78 |
| LeuMPs |
3 [0–15] (0.03%) |
4 [0–792] (0.07%) |
0.14 |
20 [0–44] (0.2%) |
8 [0–2972] (0.1%) |
0.75 |
| Procoagulant MPs | ||||||
| TF positive |
107 [0–967] (1%) |
81 [0–3980] (1%) |
0.62 |
93 [0–1164] (0.8%) |
126 [0–1268] (2%) |
0.86 |
| Fibrin positive |
101 [0–1125] (1%) |
40 [0–633] (0.7%) |
0.15 |
238 [0–411] (2%) |
41 [0–930] (0.6%) |
0.08 |
| Tumor MPs | ||||||
| Podoplanin positive |
20 [0–98] (0.3%) |
40 [0–6456] (0.7%) |
0.26 |
96 [0–4060] (0.8%) |
85 [0–988] (1%) |
0.5 |
| MUC1 positive |
0 [0–57] (0%) |
15 [0–6673] (0.3%) |
0.02 |
0 [0–11] (0%) |
0 [0–985] (0%) |
0.1 |
| CEA positive |
0 [0–23] (0%) |
20 [0–1850] (0.4%) |
0.002 |
0 [0–284] (0%) |
25 [0–4497] (0%) |
0.06 |
| CA19-9 positive |
0 [0–0] (0%) |
21 [0–4337] (0.4%) |
0.0009 |
0 [0–786] (0%) |
46 [0–777] (0.7%) |
0.64 |
(a) median (range); (b) percentage of MPs’ subtypes out of median total MPs; p<0.05 is considered as significant; Total MPs = Annexin V+ MPs; PMPs = platelet-derived MPs (Annexin V+/CD41+); EMPs = endothelial-derived MPs (Annexin V+/CD31+/CD41-);
EryMPs = erythrocyte-derived MPs (Annexin V+/ CD235a+); LeuMPs = leukocyte-derived MPs (Annexin V+/CD11b+/CD235a-); TF positive = Annexin V+/TF+;
Fibrin positive = Annexin V+/Fib+; MUC positive = AnnexinV+/MUC+; CEA positive = AnnexinV+/CEA+; CA 19-9 positive = Annexin +/CA19-9 +.