Extended Data Figure 1.

a. The distribution of CT-values for the RT-qPCR+ samples tested during the ZiBRA journey in Brazil (n=181 samples; median CT = 35.96). b. shows the distribution of the temporal lag between the date of onset of clinical symptoms and the date of sample collection of RT-qPCR+ samples (median lag = 2 days). Red dashed lines represent the median of the distributions. (c) Validation of sequencing approaches. A phylogeny of the ZIKV Asian genotype estimated using PhyML³⁷ is shown. The expanded clade highlighted in blue contains the WHO reference ZIKV sequence¹⁹ (accession number KX369547), which was generated using Illumina MiSeq. Sequences generated using MinION chemistries R9.4 2D, R9.4 1D, R9 1D, R9 2D and R7.3 2D contain no nucleotide differences and hence were also placed in this clade. Scale bars represent expected nucleotide substitutions per site (s/s). Am-ZIKV=American Zika virus lineage.