Figure 1. FIGURE 1: Sequence alignment of AAC(6’)-Im and AAC(6’)-Ie.

The secondary structure assignment for AAC(6’)-Im is shown above, and the identity (*) and similarity (:) is indicated below each block of sequence. Amino acids involved in kanamycin A binding are shaded blue for those which are common to both enzymes, red for amino acids which interact with kanamycin A only in AAC(6’)-Im, and yellow for amino acids which interact with kanamycin A only in AAC(6’)-Ie 12. The AAC(6’)-Im enzyme is one residue shorter than AAC(6’)-Ie, so for the sake of simplicity, the AAC(6’)-Im sequence numbering begins at Met2 so that the sequence numbering is the same for both enzymes.