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. 2017 Dec 8;3(12):eaao5520. doi: 10.1126/sciadv.aao5520

Fig. 3. Experiment design, opsin characterization, and demonstration of photocurrent and firing modulation via TRUE focusing.

Fig. 3

(A) An AAV vector was used to stereotaxically deliver the bReaChES transgene to the mPFC. (B) Characterization of normalized photocurrent response versus light intensity. The average maximum photocurrent across the 10 cells studied was 1047 pA. (C) Diagram illustrating the experimental scheme used to demonstrate the ability of TRUE focusing to elicit action potentials through 800-μm-thick living mouse brain tissue. (D) Representative traces demonstrating elicited photocurrent and membrane voltage changes achieved with and without TRUE focusing.