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. Author manuscript; available in PMC: 2018 Dec 7.
Published in final edited form as: Mol Cell. 2017 Nov 5;68(5):835–846.e3. doi: 10.1016/j.molcel.2017.10.016

Figure 1. Domain structure and dynamics of Ragulator.

Figure 1

(A) Schematic diagram of the domain structures of Ragulator (Lamtor1-5). The roadblock domains are labeled. The boundaries of the constructs we used for crystallization are highlighted in red. (B) The purified full length and truncated Ragulator were analyzed by SDS-PAGE. Lane 1: Full-length Ragulator; Lane 2: truncated Ragulator for crystallization. (C) Deuterium uptake data for full-length Ragulator (Lamtor1-5). HDX- MS data are shown in heat map format where peptides are represented using rectangular strips above the protein sequence. Absolute deuterium uptake after 10s, 1m and 5m is indicated by a color gradient below the protein sequence.