(A–B) Following DEAB treatment from 24–52 hpf (B), the expression area of meis3 in the vagal (vg) region (yellow arrow) and foregut tissue (white arrow) is diminished when compared with control larvae (A).
(C–D) Following heat shock induction, dnRaraa-GFP+ (D) larval fish exhibit a reduced expression domain of meis3 along the foregut (arrowhead) and vagal region, when compared to heat shock controls.
(E–F) Following RA incubation from 24–48 hpf (F), the hindbrain expression of meis3 was rostrally expanded (brackets), as well as the foregut (arrow), when compared with control larvae (E).