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. Author manuscript; available in PMC: 2018 Dec 7.
Published in final edited form as: Mol Cell. 2017 Nov 5;68(5):993–1005.e9. doi: 10.1016/j.molcel.2017.10.019

Figure 3. The tRNA methyltransferase complex TRMT6/61A catalyzes a subset of m1A methylation in mRNA.

Figure 3

(A) Motif analysis revealed a GUUCRA tRNA-like consensus for a group of m1A in mRNA, E-value=4.8e-009.

(B) Pie chart showing the percentage of m1A sites in each non-overlapping segment. Comparing to the non-motif m1A sites in mRNA, these sites are evenly distributed in the transcriptome.

(C) TRMT6/61A specifically targets m1A within the consensus sequence, while doesn’t work on m1A in non-motif sequence of the T-loop nor m1A at the 9th position of tRNA, p-value <0.005.

(D) Representative views of three mRNA targets of TRMT6/61A. The predicted RNA secondary structures are also shown, revealing a conserved stem-loop structure that harbors the GUUCRA motif.

(E) An example of non-motif mRNA m1A site, showing similar mismatch rates in the control and TRMT6/61A knock-down samples.

See also Figure S4.