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. Author manuscript; available in PMC: 2018 Dec 7.
Published in final edited form as: Cell Stem Cell. 2017 Nov 22;21(6):761–774.e5. doi: 10.1016/j.stem.2017.09.008

Figure 1. Ascl1+ and Neurog1+ Progenitors are Neuronally Specified until Injury Unlocks a Multipotent State.

Figure 1

(A) IHC of major cell types of the OE. OMP (Red) – Mature OSN; Tuj1 (Green) – Immature OSN; TRPM5 (White) – Microvillar cell; apical Sox2 (Purple) – Sus cell; Sox9/Aqp5 (Cyan) – D/G cells; basal Sox2+ (Cyan)/CK14- (Gold) – GBCs; CK14 (Gold) – HBCs. (B) Stem cell hierarchical model of olfactory epitheliopoiesis. (C) IHC of clones generated by Ascl1-CreER GBCs in uninjured, 14 days post-OBX, and 14 days post-Methimazole conditions. The arrowheads indicate non-neuronal Sus cells, while the arrow points to a GBC. The scale bar in the microvillar panel equals 10 μm and applies only to the panel it is in. (D) Counts of total cells of each type identified under each type of Ascl1 lineage trace, depicted as a percentage of total traced cells across replicates (n = 6). (E) IHC of clones generated by each type of Neurog1-CreER GBCs in all three conditions. The scale bar equals 10 μm and applies to all other panels. (F) Counts of total cells of each type identified under each type of Neurog1 lineage trace, depicted as before (n = 6). * p < 0.05, Kruskal-Wallis ANOVA on Ranks. Unless otherwise noted, comparisons are to uninjured controls (Nml). See also Figure S1.