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Immunocytochemical analysis of irradiation-induced repair foci in RNF168-deficient fibroblasts and lymphoblastoid cell lines. Detection of γH2AX foci (second column) and 53BP1 foci (third column) in reference ADP fibroblasts (upper panel) compared to patient fibroblasts F591 and F592 (middle and bottom panel) at 1 h after 6 Gy irradiation. DAPI staining and merged pictures are shown in the outer columns as controls for intracellular localization.
