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. 2017 Dec 4;11:112. doi: 10.3389/fnana.2017.00112

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Copyright © 2017 Larsen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) The inclusion line (green line) for the CP/MZ is drawn according to the outer pial surface, whereas the exclusion line (red line) for the CP/MZ is made at the interface between the CP/MZ and the SP. (B) After the cells in the CP/MZ are counted within optical disectors for this zone, the former exclusion line of the CP/MZ becomes the inclusion line for the underlying SP and a new exclusion line is drawn at the interface between the SP and the IZ. (C,D) The same procedure is applied to the IZ and the SVZ/VZ, thus ensuring that no areas (and thus no cells) are omitted or counted twice due to inconsistent delineation.