Fig. 7.

Multiple defects in Vγ4 and Vγ6 cells in HEB^ko FTOCs. a Representative FACS plots showing the frequencies of Vγ subsets among all γδ T cells in WT and HEB^ko d7 FTOCs. b Gating strategy for Vγ6 (Vγ5/1⁻Vγ4⁻CD3⁺TCRγδ⁺). c Quantification of frequencies of Vγ subsets among total γδ T cells in WT and HEB^ko d7 FTOCs. d mRNA levels of Vγ transcripts in γδ T cells sorted from WT and HEB^ko d7 FTOCs, as determined by qRT-PCR. Levels are relative to β-actin. e Intracellular expression of RORγt vs. surface CD73 in mature Vγ4 (CD3⁺TCRγδ⁺Vγ4⁺CD24⁻) from WT FTOCs, and mature Vγ6 (CD3⁺TCRγδ⁺Vγ5/4/1⁻CD24⁻) cells from WT and HEB^ko FTOCs. f Quantification of the frequencies of RORγt⁺ cells among CD24⁻CD73⁺ Vγ6 cells and CD24⁻CD73⁻Vγ6 cells from WT and HEB^ko FTOCs. All plots are gated on the CD3⁺TCRγδ⁺ population. Numbers in FACS plots indicate frequency within each gate. Data are representative of at least three independent experiments with at least 3 mice per group. Center values indicate mean, error bars denote s.e.m. p-values were determined by two tailed Student's t-test. ND = not done (due to low cell number). NS = not significant