FIGURE 4.

Verification of the interaction between ROP16 and Dnaja1 by co-immunoprecipitation. (A) Co-transfection of Myc-mROP16 and Dnaja1-GFP expressing plasmids, as well as control plasmids in different combination, into HEK293T cells and whole cell lysates (WCL) were probed with anti-Myc, anti-GFP, and anti-GAPDH (internal control). Subsequently the lysates were precipitated with an anti-Myc antibody and probed with an anti-GFP antibody to check the precipitation of Dnaja1-GFP. (B,C) Interaction between Dnaja1 and Predomain (B) or Kinase domain (C) of ROP16 determined by Co-IP, similar to (A).