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. 2017 Dec 8;7:17233. doi: 10.1038/s41598-017-17371-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Knockout of HuR reduces GM-CSF mRNA in CD4⁺ T cells. Naive WT and HuR KO CD4⁺ T cells were isolated as described in Materials and Methods and cultured with anti-CD3 and anti-CD28 stimulation in Th0 and Th17 cell polarizing conditions. Expression of GM-CSF mRNA (a,b) and protein (c–e) in Th0 and Th17 cells was measured by qRT-PCR, flow cytometry, and ELISA, respectively. Knockout of HuR reduced the GM-CSF mRNA and protein levels in Th0 and Th17 cells compared with WT counterparts. Results in (a,b,d and e) represent the summary of three independent experiments. The representative of flow analysis was shown in (c). **p < 0.01, ***p < 0.001.