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. 2017 Dec 8;7:17233. doi: 10.1038/s41598-017-17371-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MiR-466i overexpression reduced GM-CSF expression in activated macrophages. RAW264.7 cells were transfected with miR-466i mimics or scramble control at a final concentration of 10 nM, and 24 hours later after LPS stimulation (100 ng/ml), the cells were harvested. (a) Expression of GM-CSF and IL-6 mRNA levels were measured by qRT-PCR analysis and normalized by GAPDH. (b,c) GM-CSF protein expression levels were measured by flow cytometry. Data in (a) are a summary of three individual experiments. The representative flow cytometric analysis is shown in (b) and (c).