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. 2017 Dec 8;7:17226. doi: 10.1038/s41598-017-17491-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phenotypic changes and morphological response to different nitrogen applications. (a) number of spikelets per panicle; (b) number of secondary branches; (c) panicle length; (d) diameter of the panicle-neck internode; (e) number of vascular bundles. BVB: large vascular bundle; SVB: small vascular bundle; TVB: total vascular bundle. (f) cross-section of vascular bundles. OFA: optimized nitrogen fertilizer application; TFA: traditional nitrogen fertilizer application; * and ** indicate significant difference of the two nitrogen treatments at 0.05 and 0.01 probability levels, respectively.