Figure 2.
PrPSc detection in blood of animals showing clinical symptoms of CWD. Representative samples of whole blood (80 µL) from three white-tailed deer positive for PrPSc in obex and MRPLN as examined by IHC and presenting with poor body condition (S1, S2, S3), were analyzed by PMCA. As controls, blood from two deer from a CWD-free control herd (C1 and C2) were analyzed in parallel. Samples were subjected to three serial rounds of PMCA and the results of rounds 2 and 3 are shown. The PRC1 anti-PrP antibody was used for western blotting immunodetection. All samples were treated with PK, except the transgenic mice normal brain homogenate (NBH), used as migration control. Numbers in the right indicate the position of molecular weight markers.