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. 2017 Dec 8;7:17241. doi: 10.1038/s41598-017-17090-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Blind study of PrP^Sc detection in blood of CWD-infected, but asymptomatic animals. Representative samples of whole blood (200 µL) from asymptomatic white-tailed deer, were collected from either CWD-free or CWD-infected herds. Samples analyzed included 49 animals that were positive for PrP^Sc-staining in both the MRPLN and the obex via IHC (B + LN + ), 34 animals that were positive for PrP^Sc-staining only in the MRPLN via IHC (B-LN + ) and 10 that were negative in both brain and MRLPN. The figure shows representative samples from 12 B + LN + , 5 B-LN + and 3 B-LN- (Neg). The entire set of samples was analyzed independently by two different investigators in duplicate. Top and bottom panels show the results from the two different investigators which developed their western blots using two distinct anti-PrP antibodies (PRC1 and 6H4). Samples were subjected to three serial rounds of PMCA and the results obtained in the third round are shown. All samples were treated with PK, except the transgenic mice normal brain homogenate (NBH), used as migration control. Numbers in the right indicate the position of molecular weight markers.