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. 2017 Dec 8;7:17211. doi: 10.1038/s41598-017-17620-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hotspot analysis of human TSLPR. Panel A shows the TSLP:TSLPR interface as observed in the TSLP:TSLPR: IL-7Rα crystal structure. TSLP is shown in yellow cartoon representation, TSLPR in grey. Selected protein residues are shown in ball-and-stick, among which hotspot residues Arg149, Arg150 and Arg153 as identified by PredHS. Panel B shows FTMap probes in yellow ball-and-stick representation on the TSLPR surface. The two clusters are composed of the probes isobutanol, cyclohexane, dimethyl ether, ethanol, phenol, isopropanol, acetonitrile, methylamine, N,N-dimethylformamide, acetaldehyde, acetamide, ethane, acetone, urea and benzene.