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. 2017 Dec 8;7:17229. doi: 10.1038/s41598-017-17396-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Examples of EPR spectra of the C-terminal residues of EcMscL. X-band EPR spectra of underlabelled and fully spin-labelled A110C, E119C and Q132C mutants showing increased spin mobility at the top and the bottom of the A110–S136 segment of the helical bundle and reduced mobility in the middle of the A110–S136 segment. All spectra were obtained using loop-gap resonator with the microwave power of 2 mW and field modulation of 1 G.