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. 2017 Dec 8;7:17230. doi: 10.1038/s41598-017-17442-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of NK-cell related antigens. (a) Expression of NK receptors in hu-PB-NK cells in NOG-IL-15 Tg mice. Freshly isolated hu-PB-NK cells or in vivo-expanded NK cells in NOG-IL-15 Tg mice at 6 to 8 weeks after transfer were used for analyses of NK cell receptors, NKG family molecules, natural cytotoxicity receptors (NCRs), and killer cell immunoglobulin-like receptor (KIR) molecules. Expression of CD57 and CD8, maturation markers for NK cells, was also examined. The means ± SD of each fraction are shown (n = 4). Student’s t-test was performed to assess statistical significance (*p < 0.05). (b) Expression of T-bet and Eomesodermin (Eomes) in human NK cells. The fresh PB-NK cells and in vivo-expanded NK cells in NOG-IL-15 Tg mice were derived from different donors. A representative result is shown.