Figure 6.
Determination of intracellular production and accumulation of PpIX after treatment with dissolved PMVE/MA-ES electrospun nanofibers. Cells were treated for 24 h with a gradient of 5-ALA concentrations and a dissolution of empty or 5-ALA-loaded PMVE/MA-ES nanofibers (6 mg/mL in PMVEMA-ES, thus 1 mg/mL of 5-ALA). No PpIX was detected for empty nanofiber treatments, so not included in graph. Samples were excited at 412 nm and emission values taken at 665 nm. Results are expressed in average a.u. (±sd) obtained from three different experiments performed by triplicate.