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. 2017 Dec 8;8:1998. doi: 10.1038/s41467-017-02295-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Validation of BaseScope probes in cell lines. a Schematic of BaseScope technology. Binding of two custom-designed ‘Z’ probes to target mRNA directs binding of a signal amplification tree, allowing visualization of point mutations by FastRed. b, c Representative images of the validation of the KRAS G12V probeset b and BRAF V600E probeset c In both cases a wild-type cell line and a homozygous mutant cell line are shown, with a negative control probe (dapB), a positive control probe (POLR2A), the wild-type probe and the mutant probe. Probe binding is visualized as punctate red dots. Scale bars represent 50 micron and 10 micron (inset)