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. 2017 Dec 8;8:2009. doi: 10.1038/s41467-017-02221-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Hippocampal silencing of zDHHC3 abolishes HFD-dependent learning and memory impairment. a Time course (left) of LTP at CA3-CA1 synapses in hippocampal organotypic slices transfected with plasmid-encoding for control shRNA or zDHHC3 shRNA and treated with vehicle (VEH) or IPA for 24 h. Results are expressed as percentages of baseline EPSC amplitude (=100%). Insets (top) show representative EPSC at baseline (1) and during the last 5 min of LTP recording (2). On right, mean LTP values during the last 5 min (n = 7 for each group; statistics by two-way ANOVA and Bonferroni post hoc). b Preference for the novel object of mice fed SD or HFD and injected with lentiviral particles harboring control shRNA (LV-shCTR) or shRNA against zDHHC3 (LV-shzDHHC3) (n = 9 for each group; statistics by two-way ANOVA and Bonferroni post hoc). c Latency to reach the platform (n = 9 for each group; significance is indicated for LV-shCTR_HFD vs. all other groups; statistics by two-way ANOVA and Bonferroni post hoc). d Time spent in the four quadrants during probe test. NE is the target quadrant (n = 9 for each group; statistics by two-way ANOVA and Bonferroni post hoc). e Palmitoylated GluA1 (left, top) and total immunoprecipitated protein (left, bottom) in hippocampi. Densitometry (right) of palmitoylated GluA1/total immunoprecipitated GluA1 ratio (n = 3 per each group; statistics by two-way ANOVA and Bonferroni post hoc). f Immunoblots of pGluA1 Ser⁸⁴⁵ and densitometry of pGluA1 Ser⁸⁴⁵ normalized to both the total GluA1 and tubulin (n = 5 mice per group; statistics by two-way ANOVA and Bonferroni post hoc). g Time course (left) of LTP at CA3-CA1 synapses in hippocampal organotypic slices transfected with plasmids encoding for GluA1 WT or GluA1 C585S/C811S. Results are expressed as percentages of baseline EPSC amplitude (=100%). Insets (top) show representative EPSC at baseline (1) and during the last 5 min of LTP recording (2). On right, mean LTP values during the last 5 min (n = 12 for each group; statistics by two-way ANOVA and Bonferroni post hoc). Data are expressed as mean ± SEM *p < 0.05; **p < 0.01; ***p < 0.001; n.s. not significant. See also Supplementary Fig. 4