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. 2017 Dec 8;7:17235. doi: 10.1038/s41598-017-17619-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RNA quality analysis and gene expression. (a) Capillary electrophoresis gel images and electropherograms show the range of RNA quality from representative nodule tissues (1–3). Corresponding DV₂₀₀ metric values (and accompanying RNA Quality Number, RQN) were: sample 1 = 93.5%, (7.4); sample 2 = 80.5%, (3.3); sample 3 = 38.7%, (1.0). (b) Tukey box and whiskers graph displaying average DV₂₀₀ metrics for either nodule or synovial membrane RNA samples, which undergo identical sample handling; ***p = 0.0005. (c) Sample DV₂₀₀ metric values for nodule-derived RNA are positively correlated with the levels of combined endogenous control gene (GAPDH and HPRT1) expression (r = 0.9087; p < 0.0001). Capillary electrophoresis gel images shown in (a) for samples 1–3 are from an original results figure (pdf file), contrast-enhanced to highlight fragmented RNA; Each image was then individually cropped and uniformly resized. Electropherograms shown are black and white versions from an original results figure (pdf file) with no further manipulation. Full versions of these original figures are presented in Supplementary Figures S1 and S2.