Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Dec 8;7:17235. doi: 10.1038/s41598-017-17619-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Influence of fragmented RNA and data normalisation on gene expression. (a) A series of RNA samples (A–E) containing set proportions of intact THP-1 cell RNA, spiked with increasing proportions of highly-fragmented nodule RNA. Corresponding RNA integrity number (RIN) values for the samples were: A: 8.3; B: 8.5; C: 8.1; D: 4.6; E: 2.5. Using dPCR, combined and averaged absolute measures of GAPDH and HPRT1 endogenous control gene expression positively correlate with both the proportion of pre-RT intact RNA (▴; r = 0.9891; p = 0.0014) and the DV₂₀₀ metrics (Δ; r = 0.9729; p = 0.0053). (b) Absolute (▫) and normalised values () of PRTN3 expression in samples (A–D), with the 95% CI for the copies/pg RNA. The dashed line marks the upper 95% CI limit for sample A. Normalisation was applied according to the calculated endogenous control gene normalisation factors of A: 1; B: 1.49; C: 2.74; D: 6.12; E (not displayed): 3232.

Inline graphic — Influence of fragmented RNA and data normalisation on gene expression. (a) A series of RNA samples (A–E) containing set proportions of intact THP-1 cell RNA, spiked with increasing proportions of highly-fragmented nodule RNA. Corresponding RNA integrity number (RIN) values for the samples were: A: 8.3; B: 8.5; C: 8.1; D: 4.6; E: 2.5. Using dPCR, combined and averaged absolute measures of GAPDH and HPRT1 endogenous control gene expression positively correlate with both the proportion of pre-RT intact RNA (▴; r = 0.9891; p = 0.0014) and the DV₂₀₀ metrics (Δ; r = 0.9729; p = 0.0053). (b) Absolute (▫) and normalised values () of PRTN3 expression in samples (A–D), with the 95% CI for the copies/pg RNA. The dashed line marks the upper 95% CI limit for sample A. Normalisation was applied according to the calculated endogenous control gene normalisation factors of A: 1; B: 1.49; C: 2.74; D: 6.12; E (not displayed): 3232.