Fig. 3.

GADD45B epigenetically regulates Kv1.1 expression. a Top: working hypothesis. Bottom: Gadd45b expression is decreased in ThGrin1KO VPM neurons. Student’s t-test, *P < 0.05, **P < 0.01. b DNA methylation of the Kcna1 promoter region is increased in Gadd45b^−/− VPM neurons. Top: schematic representation of the Kcna1 promoter region. Bottom: bisulfite sequencing results. Kruskal–Wallis test, **P < 0.01. c In situ hybridization shows decreased Kcna1 expression in Gadd45b^−/− VPM (n = 3). Red arrowheads show the labeling in VPM. Scale bar: 100 μm. d Gadd45b^−/− VPM neurons are hyperexcitable. Student’s t-test, **P < 0.01. e Summary of the findings